Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos.
نویسندگان
چکیده
Interference of the Hedgehog (Hh) signaling pathway by cyclopamine leads to abnormal embryonic development. We monitor this dynamical drug effect in zebrafish embryos with highly precise microenvironment control using an integrated microfluidic device. This chip-based platform, which is programmable and automated, greatly facilitates the accuracy and reproducibility of the in vivo assays.
منابع مشابه
Miniaturized Embryo Array for Automated Trapping, Immobilization and Microperfusion of Zebrafish Embryos
Zebrafish (Danio rerio) has recently emerged as a powerful experimental model in drug discovery and environmental toxicology. Drug discovery screens performed on zebrafish embryos mirror with a high level of accuracy the tests usually performed on mammalian animal models, and fish embryo toxicity assay (FET) is one of the most promising alternative approaches to acute ecotoxicity testing with a...
متن کاملInvestigation of Toxicological Effects of di-2-ethylhexyl with Fish Embryo Test (FET) in Zebrafish (Danio rerio)
Di(2-ethylhexyl)phthalate (DEHP) is a plasticizer in a variety of consumer products. Given the adverse toxicity effects of DEHP on living organisms, its high consumption and dispersal has been caused a serious concern. . Nowedays, the Fish Embryo Test (FET) method is widely used among the conventional methods of toxicology .The aim of the this study was to determine the acute toxicity of Di(2-e...
متن کاملIntegrated chip-based physiometer for automated fish embryo toxicity biotests in pharmaceutical screening and ecotoxicology.
Transgenic zebrafish (Danio rerio) models of human diseases have recently emerged as innovative experimental systems in drug discovery and molecular pathology. None of the currently available technologies, however, allow for automated immobilization and treatment of large numbers of spatially encoded transgenic embryos during real-time developmental analysis. This work describes the proof-of-co...
متن کاملNumerical Study of Droplet Generation Process in a Microfluidic Flow Focusing
Microfluidic flow focusing devices have been utilized for droplet generation on account of its superior control over droplet size. Droplet based microfluidics addressed many scientific issues by providing a novel technological platform for applications such as biology, pharmaceutical industry, biomedical studies and drug delivery. This study numerically investigated the droplet generation proce...
متن کاملDesign and Implementation of an Automated Dispensing Device for Reducing Methadone Therapy Misuse
Introduction: A variety of (deliberate or inaccurate) errors in drug rehabilitation clinics may occur due to use of addictive drugs. Automated drug distribution systems play a significant role in reducing drug medication errors. Method: Firstly, according to the addiction rehabilitation centers requests, the design and construction of the device was performed. The prototype systems were examin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 50 8 شماره
صفحات -
تاریخ انتشار 2014